Evidence-Based Complementary and Alternative Medicine

Original Article

Bacopa monniera Attenuates Scopolamine-Induced Impairment of Spatial Memory in Mice

Table 1

Regimen for administration of vehicle and pharmacological agents.


Treatment	Group	Figure	Administration of vehicle and pharmacological agents
Treatment	Group	Figure	Acquisition trials (Days 1–6)	Retrieval trial (Day 7)

Control	I (n = 7)	Figures 1A–F, 2(a), and 2(b)	NS (10 mL kg⁻¹ PO) 60 min before + NS (10 mL kg⁻¹ i.p.) 30 min before	NS (10 mL kg⁻¹ PO) 60 min before + NS (10 mL kg⁻¹ i.p.) 30 min before
5% Tween 80 per se	II (n = 7)	Figures 1A–F, 2(a), and 2(b)	Tween 80 (10 mL kg⁻¹ PO) 60 min before + NS (10 mL kg⁻¹ i.p.) 30 min before	Tween 80 (10 mL kg⁻¹ PO) 60 min before + NS (10 mL kg⁻¹ i.p.) 30 min before
Scopolamine anterograde	III-V (n = 7/group)	Figures 1(b)–1(d)	Tween 80 (10 mL kg⁻¹ PO) 60 min before + Sco (1 mg kg⁻¹ i.p., 0.5 mg kg⁻¹ i.p., 0.1 mg kg⁻¹ i.p.) 30 min before	Tween 80 (10 mL kg⁻¹ PO) 60 min before + NS (10 mL kg⁻¹ i.p.) 30 min before
Scopolamine retrograde	VI–VII (n = 7/group)	Figure 2(b)	Tween 80 (10 mL kg⁻¹ PO) 60 min before + NS (10 mL kg⁻¹ i.p.) 30 min before	Tween 80 (10 mL kg⁻¹ PO) 60 min before + Sco (0.5 mg kg⁻¹ i.p., 0.1 mg kg⁻¹ i.p.) 30 min before
Bacopa monniera + Scopolamine 0.5 mganterograde	VIII (n = 7)	Figure 2(b)	bm (120 mg kg⁻¹ PO) 60 min before + Sco (0.5 mg kg⁻¹ i.p.) 30 min before	Tween 80 (10 mL kg⁻¹ PO) 60 min before + NS (10 mL kg⁻¹ i.p.) 30 min before
Bacopa monniera + Scopolamine 0.1 mganterograde	IX (n = 7)	Figure 2(b)	BM (120 mg kg⁻¹ PO) 60 min before + Sco (0.1 mg kg⁻¹ i.p.) 30 min before	Tween 80 (10 mL kg⁻¹ PO) 60 min before + NS (10 mL kg⁻¹ i.p.) 30 min before
Bacopa monniera + Scopolamine 0.5 mgretrogarde	X (n = 7)	Figures 2E and F	Tween 80 (10 mL kg⁻¹ PO) 60 min before + ns (10 mL kg⁻¹ i.p.) 30 min before	BM (120 mg kg⁻¹ PO) 60 min before + Sco (0.5 mg kg⁻¹ i.p.) 30 min before
Bacopa monniera−per se	XI (n = 7)	Figures 1(a), 2(b)	BM (80 mg, 120 mg kg⁻¹ PO) 60 min before	BM (80 mg, 120 mg kg⁻¹ PO) 60 min before

BM: Bacopa monniera standardized extract suspension in 5% Tween 80, NS: normal saline, n: number of mouse; Sco: scopolamine solution in normal saline; Tween 80: 5% v/v Tween solution in normal saline.