Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site

Figure 1

PCR primer sets for the cap site hunting method analysis of the Aig1l gene. The cap site hunting method allowed detection of the transcriptional start site of the gene. Horizontal arrows indicate positions of the primer pairs. In the first PCR, we prepared primer sets for the brain (RDT1 and R4) and skeletal muscle (RDT1 and R3). In the nested PCR, we prepared primer sets for the brain (RDT2 and R2) and skeletal muscle (RDT2 and R1). The vertical arrow points to the transcriptional start site. Underlining shows the start codon atg. Italic letters designate the synthetic rOligo sequence ligated to the mRNA.