Full-Length Sequence of Mouse Acupuncture-Induced 1-L (Aig1l) Gene Including Its Transcriptional Start Site
Figure 3
Tissue distribution and effect of EA on Aig1l gene expression. (a) Tissue distribution of Aig1l as detected by northern blot analysis in the normal sensitivity mode. (b) Tissue distribution of Aig1l as detected by real-time quantitative PCR analysis (n = 5). (c) Semiquantitative RT-PCR analysis of Aig1l gene expression in skeletal muscle after EA. Lanes are as follows: C: no EA stimulation (control); 0: EA-treated sample just after stimulation; 1, 3 and 24: EA-treated samples at 1, 3 and 24 hours after stimulation, respectively; NC: negative PCR control containing no cDNA template in the PCR mixture; G3PDH served as a loading control. The following tissues expressed the Aig1l gene in (a) and (b): brain, B; muscle, M; heart, H; lung, Lu; spleen, S; liver, L and kidney, K. NC indicates negative control.
