Novel Lipolytic Enzymes Identified from Metagenomic Library of Deep-Sea Sediment

Figure 2

Multiple alignments of the conserved motifs of the ORFs isolated from metagenomic library with the homologs of each family or group. (a) Family V including EM3L1 and EM3L6: YP_003395264, an 𝛼/𝛽 hydrolase fold protein from Conexibacter woesei DSM 14684; ZP_01697334, an 𝛼/𝛽 hydrolase fold from Bacillus coagulans 36D1; NP_767479, a hypothetical protein bll0839 from Bradyrhizobium japonicum USDA 110; ZP_06812025, an 𝛼/𝛽 hydrolase fold protein from Geobacillus thermoglucosidasius C56-YS93. (b) Family V including EM3L3: AAK07450, a triacylglycerol acyl hydrolase from Moritella marina; YP_001530963, an 𝛼/𝛽 hydrolase fold from Desulfococcus oleovorans Hxd3; YP_001188192, an 𝛼/𝛽 hydrolase fold from Pseudomonas mendocina ymp (YP_001188192). (c) EstD2 family including EM3L2: ZP_01915829, a hypothetical protein from Limnobacter sp. MED105; ADN26553, an EstD2 from uncultured bacterium; ZP_00952831, a hypothetical protein from Oceanicaulis alexandrii HTCC2633; YP_003594738, a hypothetical protein from Caulobacter segnis ATCC 21756. (d) New group including EM3L4: ZP_01463024, an LpqC from Stigmatella aurantiaca DW4/3-1; YP_004018523, a lipoprotein from Frankia sp. EuI1c; ZP_06968499, a putative lipoprotein from Ktedonobacter racemifer DSM 44963; ZP_06502439, a conserved hypothetical protein from Micrococcus luteus SK58. (e) Family I including EM3L7: ACJ13070, a lipase from uncultured bacterium; (ACJ13070); XP_390196, a hypothetical protein from Gibberella zeae PH-1; XP_504639, a YALI0E31515p from Yarrowia lipolytica. Triangles and squares represent the residues involved in the formation of the catalytic triad and the oxyanion hole, respectively, and the conserved pentapeptide motifs are boxed.