Novel Lipolytic Enzymes Identified from Metagenomic Library of Deep-Sea Sediment
Figure 3
Expression of esterase/lipase genes. (a) Expression analysis of EM3L1, EM3L2, and EM3L3; (b) expression analysis of EM3L4, EM3L6, and EM3L7 with co-expression of the GroEL-GroES chaperone genes at 16°C; (c) purified enzymes. The bands corresponding to the proteins are indicated by arrows. Lane M: molecular mass standards, lane T: total cell lysate, lane S: soluble fraction, lane P: insoluble fraction, lane 1: purified EM3L1, lane 2: purified EM3L2, lane3: purified EM3L3, lane 4: purified EM3L4, and lane 5: purified EM3L6.
