Novel Lipolytic Enzymes Identified from Metagenomic Library of Deep-Sea Sediment

Table 1

Primers used in the cloning of lipolytic enzyme-encoding genes.

Gene	Primer*
EM3L1	5′-TTTGGAGGCATATGCTTATCCCTTCCGATGGTCTGGAAC-3′
	5′-CTGGTGTCCTCGAGGTTTGCCAAAAAACGGCCGTATATC-3′
EM3L2	5′-TGATAAATCATATGAACAGAATGACAATAGGCTTCTC-3′
	5′-CCTTTTAGCTCGAGATTATTGTCTTGCAACCAGGATT- 3′
EM3L3	5′-TAGCTGCACATATGATCCTTTTTAGCTTGGTCGGTGTC-3′
	5′-AAAGTTGGCTCGAGTGTCTTAATCCCAAGAAAATTCAAG-3′
EM3L4	5′-GAGAAGTGAACCGGGGGACATATGACTGGTAGAATTG-3′
	5′-CAAAAGCGCCCCTCGAGCGGCTGCTCCTCC-3′
EM3L6	5′-AGGAGAAGCATATGAAATGCATCCCATCAGACGGCC-3′
	5′-GTTGCAGACTCGAGATCATTATTCGAGCCTAATTCCTC-3′
EM3L7	5′-CGGAGTGAAAGCATATGACTTATCCGATTGTGCTC-3′
	5′-GTATTACCTCTCGAGGTTAAGGTAGTTGTTCCGCGATTC-3′

*Underlined bases in the primers indicate the restriction enzyme recognition site (NdeI/XhoI).