Novel Lipolytic Enzymes Identified from Metagenomic Library of Deep-Sea Sediment

Table 2

Characteristics of the purified lipolytic enzymes.

Enzyme	Temperature^a; (optimum) (°C)	Activation energy; (kcal/mol)	pH^b; (optimum)	Substrate^c; (optimum)	Specific activity; (U/mg)
EM3L1	15–50 (35)	3.6	7.5–9.5 (8.5)	C2–C10 (C6)	558.2
EM3L2	15–40 (30)	8.6	7.0–10.0 (8.5)	C6 (C6)	1.7
EM3L3	15–40 (35)	3.5	7.0–10.0 (8.5)	C2–C10 (C6)	29.7
EM3L4	20–50 (35)	10.6	7.0–10.0 (7.5)	C16–C18 (C16)	5.3
EM3L6	15–40 (35)	4.1	7.5–9.0 (8.5)	C2–C10 (C6)	23.3

^aThe temperature range is the temperatures at which the activities are greater than 50% of the highest value (Figure S1). ^bpH range is the pHs at which the activities are greater than 50% of the highest value (Figure S2). ^cSubstrates are the p-nitrophenyl esters for which the enzyme shows an activity greater than 20% of the highest value (Figure S3).