Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells
Figure 5
Binding capability of rATB to diverse leukocytes. Flow cytometric analysis of lysed whole blood after incubation with rATB (500 ng/mL) in the presence or absence of sugars (50 mM). (a) Granulocytes, monocytes, and lymphocytes were identified by setting a polygonal gate in a forward scatter/sideward scatter dot plot. (b) Percentages of positively stained granulocytes, monocytes, and lymphocytes exhibit a shift toward the right in comparison with control. (c) Leukocytes were preincubated with competitive sugar (Gal) or uncompetitive sugar (Man) as indicated by arrows. Data from one of three independent experiments with similar results are shown.
