CI431, an Aqueous Compound from Ciona intestinalis L., Induces Apoptosis through a Mitochondria-Mediated Pathway in Human Hepatocellular Carcinoma Cells
Figure 3
Morphological analysis of BEL-7402 cells induced by CI431. BEL-7402 cells were grown on poly-L-lysine-coated coverslips for 24 h to allow firm attachment and treated with 50 μg/mL CI431 for certain time intervals. Cells were fixed on coverslips coated with gold and analyzed by using the KYKY-2800B SEM. The cells were untreated (a) or treated with CI431 for 12 (b), 18 (c), and 24 h (d). The untreated BEL-7402 cells showed a normal smooth surface. In contrast, the cells treated with CI431 became rounded, and the surface of the cell membrane was markedly disrupted (Scale bar = 10 μm).
