CI431, an Aqueous Compound from Ciona intestinalis L., Induces Apoptosis through a Mitochondria-Mediated Pathway in Human Hepatocellular Carcinoma Cells
Figure 6
Cytofluorometric analysis of Δψm BEL-7402 cells were seeded in 12-well plates at a density of cells/mL and treated with CI431 at doses of 20, 40, and 80 μg/mL for 24 h. After treatment with CI431 200 μL pre-warmed incubation buffer containing 0.2 μL MitoCapture was added to each well and plates were incubated for 15 min at 37°C in a 5% CO2 incubator. Then, cells were analyzed by a Fluorescence Spectrophotometer (F-4500, HITACHI, Japan). After treatment with CI431, Δψm began to decrease, and the ratio of green fluorescence intensity to red fluorescence intensity was 0.61 ± 0.07 (blank, a), 1.92 ± 0.19 (20 μg/mL, b), 5.08 ± 0.45 (40 μLgmL, c) and 8.28 ± 0.61 (80 μg/mL, d), respectively, indicating disruption of mitochondrial function.
