Ganoderma lucidum Polysaccharides Induce Macrophage-Like Differentiation in Human Leukemia THP-1 Cells via Caspase and p53 Activation
Figure 6
The effects of caspase inhibitor (Z-VAD-FMK) or p53 inhibitor (PET-α, and F3 alone or in combination with inhibitors, on the differentiation of THP-1 cells into macrophages were observed by flow cytometry analysis. (a) Caspase inhibitors (6, 20, 50 M) or (b) p53 inhibitors (5, 50, 100 M) were added to either the control or F3-treated cultures after 24 h of incubation. The percentage of CD11b and CD14 positive cells and MFI of CD11b and CD14 positive cells were shown as the mean ± SD, and individual Student's t-test was performed between F3-treated THP-1 cells and caspase inhibitor combined with F3 co-cultured groups (*, **) in comparison to F3-treated cells.
