Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo
Figure 4
The effects of DNE3 on cell viability, migration, motility, invasion, and adhesion of melanoma cells. (a) B16F10 and (b) HS68 cells were treated with DNE3 for 24 hours by MTT assay. (c) B16F10 cells were subjected to analyze for cell migration by wound healing assay. (d) Determined migration ability of B16F10 was subsequently quantified with that of control being 100% (without DNE3 for 48 h). Cells were treated with DNE3 for 24 hours and then subjected to analyze for (e and f) motility, invasion, and (g) adhesion as described in Materials and Methods. Results were statistically evaluated by using one-way ANOVA with post hoc Dunnett's test (***). Results from 3 repeated and separated experiments were similar.
