The Largest Bio-Silica Structure on Earth: The Giant Basal Spicule from the Deep-Sea Glass Sponge Monorhaphis chuni
Figure 9
Poriferan cryptochromes. (a) The deduced poriferan cryptochrome protein sequences CRY_SUBDO (Suberites domuncula (CRYPTO_SUBDO; accession number FN421335), CRY_CRAME (Crateromorpha meyeri; FN421336), and the photolyase-related protein from Aphrocallistes vastus (PHR_APHVA; AJ437143.1) were aligned with the coral (Acropora millepora) cryptochrome CRY1 (CRY1_ACRO; 145881069). Residues conserved (identical or similar with respect to their physicochemical properties) in all sequences are shown in white on black; those which share similarity in three sequences are shown in black on gray. The characteristic domains the N-terminal photolyase-related region (photolyase), and the FAD-binding domain, are marked. (b) Phylogenetic relationship of the photolyase/cryptochrome polypeptides. The sponge photolyase-related molecule (PHR_APHVA) has been aligned with related sequences; finally a rooted tree has been computed. The numbers at the nodes are an indication of the level of confidence for the branches as determined by bootstrap analysis (1000 bootstrap replicates). The scale bar indicates an evolutionary distance of 0.1 aa substitutions per position in the sequence. The following sequences have been included [“class I photolyases”]. The cryptochrome sequences from Danio rerio zcry1a (CRY1a_DARE; AB042248/AB042248), zcry1b (CRY1b_DARE; AB042249/AB042249.1), zcry2a (CRY2a_DARE; AB042250/AB042250.1), zcry2b (CRY2b_DARE; AB042251/AB042251.1), zcry3 (CRY3_DARE; AB042252/AB042252.1), zcry4 (CRY4_DARE; AB042253/AB042253.1), and (6–4) photolyase (CRY6-4_DARE; AB042254), the human photolyase (CRY_HUMAN; D83702), mouse photolyase/blue-light receptor homolog 1 (CRY1_MOUSE; AB000777) and homolog 2 (CRY2_MOUSE; AB003433), frog cryptochrome 1 (CRY_XENLA; AY049033), the photolyase from D. melanogaster (PHR1_DROME; BAA12067.1). The Arabidopsis thaliana class II photolyases (CPD photolyase; CPD_ARATH; CAA67683.1) were used as outgroup to root the tree.
