Suppression of Heregulin-β1/HER2-Modulated Invasive and Aggressive Phenotype of Breast Carcinoma by Pterostilbene via Inhibition of Matrix Metalloproteinase-9, p38 Kinase Cascade and Akt Activation
Figure 3
Effect of pterostilbene on HRG-β1-modulated wound healing. MCF-7 cells were seeded on 6-well plates, starved, and grown to confluence. A scrape wound was generated in the cell layer, and the culture was treated with pterostilbene at a concentration of 0, 5, 10 or 20 M for 30 min prior to HRG-β1 (20 ng/ml) stimulation. Then the cells on the edge of the wound were allowed 24 h to migrate across the wound. All of the results were monitored by microscopy. Relative distance of the wound width was measured and divided by the initial half-width of the wound. Results are expressed as the mean ± SE of three independent experiments. *, ** and *** versus HRG-β1-stimulated control; ### compared with serum-starved control.
