Suppression of Heregulin-β1/HER2-Modulated Invasive and Aggressive Phenotype of Breast Carcinoma by Pterostilbene via Inhibition of Matrix Metalloproteinase-9, p38 Kinase Cascade and Akt Activation
Figure 7
Effects of pterostilbene on the activation of p38 kinase, ERK1/2 and Akt in HRG-β1-stimulated cells. Serum-starved MCF-7 cells were pretreated with a variety of concentrations of pterostilbene, SB203580 (p38 kinase inhibitor, 20 M), or PD98059 (MEK inhibitor, 50 M) for 30 min and then stimulated by HRG-β1 (20 ng/ml) for 10 min. (a) Phosphorylated p38 kinase (p-38), (b) phosphorylated ERK1/2 (p-ERK1/2), (c) phosphorylated Akt (p-Akt) or MMP-9 was determined by western blotting. The native protein or β-actin was used as a loading control.
