Research Article

Ocimum gratissimum Aqueous Extract Protects H9c2 Myocardiac Cells from H2O2-Induced Cell Apoptosis through Akt Signalling

Figure 4

Effects of Ocimum gratissimum extracts on protein level of Bcl-2, tBid, Apaf-1, and Cytosolic cytochrome c (CytC). H9c2 cells were pretreated with 0, 50, and 100  g/mL OGE for 3 hrs and then treated with H2O2 for 24 hrs. After the treatments, the H9c2 cells were lyzed for immunoblotting. The protein levels of Bcl-2, Apaf-1, and cytochrome c are, respectively, indicated. -actin was used for normalization.
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