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Evidence-Based Complementary and Alternative Medicine
Volume 2011 (2011), Article ID 617438, 9 pages
http://dx.doi.org/10.1093/ecam/neq001
Original Article

Administration of Chinpi, a Component of the Herbal Medicine Ninjin-Youei-To, Reverses Age-Induced Demyelination

1Department of Neuroglia Cell Biology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan
2Center for Kampo Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan
3Tsumura Laboratory, Tsumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan
4Biomedical Research Center, Division of Morphological Science, Saitama Medical School, 38 Morohongo, Iruma-gun, Saitama, 350-0495, Japan
5Kracie Pharmaceutical, Ltd., 1-8-63 Tenmanbashi, Kita-ku, Osaka 530-0042, Japan
6Sun R&D Institute for Natural Medicine Co. Inc., 3-12-6 Ginza, Chuo-ku, Tokyo 104-0061, Japan
7Nishiogi Animal Hospital, 4-4-5 Nishiogikita, Suginami-ku, Tokyo 167-0042, Japan
8NHK Enterprises, Inc., 4-14 Kamiyama-cho, Shibuya-ku, Tokyo 150-0047, Japan
9Kudou Chiaki Hospital, 1-23-10 Omori-kita, Ota-ku, Tokyo 143-0016, Japan
10Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kamei-cho, Fujisawa-shi, Kanagawa 252-8510, Japan

Received 9 April 2009; Accepted 29 December 2009

Copyright © 2011 Nanako Sato et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The disruption of myelin causes severe neurological diseases. An understanding of the mechanism of myelination and remyelination is essential for the development of therapeutic strategies for demyelination diseases. Our previous findings indicated that the FcRγ/Fyn cascade is a potential therapeutic target for remyelination caused by the Chinese/Japanese traditional herbal (Kampo) medicine ninjin’youeito (Ninjin-youei-to, NYT), which is a hot-water extract made from 12 medicinal herbs. To identify which constituents of NYT are involved in the reversal of demyelination and to examine the potential therapeutic effect, we tested several of the chemical constituents of NYT. Here, we report that Chinpi, a constituent of NYT, upregulates the FcRγ/Fyn signaling cascade resulting in a potentially therapeutic effect against age-induced demyelination. In addition, we observed that phosphorylated (activated) FcRγ/Fyn upregulated the expression of the 21.5 kDa isoform of myelin basic protein, inducing rapid morphological differentiation, when oligodendrocyte precursor cells (OPCs) were cultured in the presence of hesperidin and/or narirutin (the major active constituents of Chinpi). These results suggest that hesperidin and narirutin participate in the FcRγ/Fyn signaling pathway in OPCs causing these cells to differentiate into myelinating oligodendrocytes.