Biochanin A, a Phytoestrogenic Isoflavone with Selective Inhibition of Phosphodiesterase 4, Suppresses Ovalbumin-Induced Airway Hyperresponsiveness

Figure 3

Effects of biochanin A (30~100 μmol/kg, p.o.) on 𝑃_enh (a), inflammatory cells (b), and cytokines (c) in sensitized mice receiving aerosolized methacholine (MCh, 6.25~50 mg/mL) 2 days after primary allergen challenge. ^#𝑃<.05, ^##𝑃<.01, and ^###𝑃<.001, compared to the nonchallenged group. *𝑃<.05, **𝑃<.01, and ***𝑃<.001, compared to the control (vehicle) group. The number of mice in each group was 10. PBS, phosphate-buffered saline; Total, total cells; Mac, macrophages; Lym, lymphocytes; Neu, neutrophils; Eos, eosinophils; IL, interleukin; TNF-𝛼, tumor necrosis factor-𝛼; TNF-𝛾, tumor necrosis factor-𝛾.

(a)

(b)

(c)