Antioxidant and Hepatoprotective Effects of Silibinin in a Rat Model of Nonalcoholic Steatohepatitis
Figure 2
Effect of silibinin on liver histology. Representative HPS staining of rat liver sections. The Control (a-CTL; ×20) group received 12 weeks of standard liquid diet and showed normal histology. The NASH (b–d NASH; ×10, ×40 and ×20, resp.) group received a high-fat liquid diet during 12 weeks and revealed severe steatosis, hepatocyte ballooning, important inflammatory cell infiltration such as monocytes (M) and neutrophils (N), necrosis (Nec), Mallory bodies (Mallory) and fibrosis. A 12-week high-fat liquid diet supplemented with silibinin–phosphatidylcholine complex (e-SIL; ×20), at a dose of 200 mg kg−1 as silibinin during the last 5 weeks, displayed mild steatosis and moderate inflammation.
