Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies
Figure 3
Proliferation of tissue lymphocytes from all groups after in vitro stimulation with LCDV. (a) Intramuscular injection; (b) hypodermic injection. Cells were harvested on day 21 and cultured for two days. Control group (vertical bar); PBS group (horizontal bar); 5 μg pEGFP-N2 group (triangular bracket); 0.1 μg pEGFP-N2-LCDV-cn0.6 kb group (pane); 5 μg pEGFP-N2-LCDV-cn0.6 kb group (wave bar); 15 μg pEGFP-N2-LCDV-cn0.6 kb group (dot). Results are shown as the mean ± S.E.M. of the OD450 values. Significant differences () were observed between the pEGFP-N2-LCDV-cn0.6 kb group and the no-injection groups, and the PBS and pEGFP-N2 groups.
