Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo

Table 1

Stimulatory effect of DsCE-II on regeneration of myeloid progenitor CFU-GM cells in bone marrow of 5-FU-treated mice.

Treatment	CFU number × 10^−2 (percentage of control)
	GM (%)	BFU-E (%)	GEMM (%)
–5-FU control	635.2 ± 42.1 (100)^a	24.8 ± 6.3 (100)	29.2 ± 4.6 (100)
+5-FU H2O	175.5 ± 17.1 (27.6)	2.7 ± 0.3 (10.9)	2.4 ± 0.4 (8.2)
Veg	205.7 ± 17.8 (32.4)*	3.1 ± 0.7 (12.5)	2.2 ± 0.4 (7.5)
DsCE-I	263.3 ± 18.7 (41.5)	2.4 ± 0.8 (9.7)	3.4 ± 1.0 (11.6)
DsCE-II	427.4 ± 49.2 (67.3)**	5.3 ± 0.9 (21.4)	3.5 ± 0.5 (11.9)
G-CSF	501.6 ± 15.7 (78.9)**	1.4 ± 0.5 (5.6)	7.3 ± 1.4 (25.0)

Mice were treated with 100 mg kg⁻¹ body weight 5-FU, after 24 h mice were force-fed with water, Dioscorea extracts (DsCE-I and II of D. batatas, 10 mg kg⁻¹ body weight), and vegetable (cucumber juice, 10 mg kg⁻¹ body weight), or injected subcutaneously with G-CSF (5 𝜇g kg⁻¹ body weight) for five consecutivedays. BMCs were harvested and subjected to colony assay as described in the ‘Methods’ section. Bone marrow of one hind limb (femur and tibia) was assessed for CFU number. Data are expressed as mean ± SD for a group of three mice. This experiment was performed three times giving analogous results. Results are presented with data pooled from two independent experiments. BFU-E: burst forming unit-erythroid; CFU:colony-forming unit, GEMM: granulocyte, erythrocyte, monocyte and megakaryocyte; GM: granulocyte and macrophage.
^aPercentage of colony number over normal (no 5-FU) controls.
Significant difference when compared with 5-FU treatment control *𝑃<.05; **𝑃<.01.