Ocimum gratissimum Aqueous Extract Induces Apoptotic Signalling in Lung Adenocarcinoma Cell A549

Figure 5

OGE enhanced protein level of Bak and diminished protein level of Bcl-2. A549 cells were treated with 0, 500, and 800 𝜇g/ml for 48 hr, and then were lyzed for the determination of protein levels by immunoblotting. (a) The expression levels of Bak and Bcl-2 were determined. GAPDH was used as control. (b) The expression levels of Bak and Bcl-2 were quantitatively expressed after being standardized to GAPDH. Data are expressed as mean ±SEM for 3 independent experiments for each concentration point. ^∗∗𝑃<.01 as compared with control.

(a)

(b)