Agaricus blazei Extract Induces Apoptosis through ROS-Dependent JNK Activation Involving the Mitochondrial Pathway and Suppression of Constitutive NF-κB in THP-1 Cells
Figure 3
Effect of ABE on expression
of apoptotic proteins. THP-1 cells were seeded at 2 × 105
cells ml−1 and were then treated with 4 mg ml−1
of ABE for the indicated time. (a) Total cell extracts resolved
by SDS–PAGE, transferred to nitrocellulose, and probed with specific antibodies
(a) anti-Bax, anti-Bad and anti-Bcl-2; (d) anti-caspase-3, anti-caspase-8, anti-caspase-9 and anti-PARP).
(b) Equal amounts of mitochondria and cytosol cell lysates were resolved by SDS–PAGE,
transferred to nitrocellulose, and probed with a specific antibody for cytochrome c.
Equal protein loading was evaluated by β-actin. The results shown are from one representative experiment
of three experiments that exhibited similar patterns. (c) Caspase (-3, -8 and -9) activities
were determined using caspase assay kits. Each point represents the mean ± SD of three independent experiments.
The significance was determined by Student's t-test ( versus vehicle
control (0)). Cyto, Cytoplasm; Mito, Mitochondria.
