Seed Oil of Brucea javanica Induces Apoptotic Death of Acute Myeloid Leukemia Cells via Both the Death Receptors and the Mitochondrial-Related Pathways
Figure 1
Cytotoxic effects of BJO on different tumor cell lines. HL-60 and U937 cells were treated with emulsifier (negative control), etoposide (10 μmol/L, positive control), or various concentrations of BJO as indicated for 6 hours. (a) Cell viabilities were determined by MTT assay. *** (b) U937 cell morphology analysis following treatment with BJO. AO/EB and Hochest3325 were used to stain cells and the cellular fluorescent changes were observed using fluorescence microscope. Results were obtained from three separate experiments. (c) Apoptotic cells were evaluated by cell cycle analysis. Percentage of cells in the subG1 phase was measured using flow cytometry as described in Materials and Method. (d) Western blotting analysis of PARP. β-actin is shown as a loading control. (e) DNA fragmentation analysis. The level of DNA fragmentation was determined as described in Materials and Method.
