Seed Oil of Brucea javanica Induces Apoptotic Death of Acute Myeloid Leukemia Cells via Both the Death Receptors and the Mitochondrial-Related Pathways

Figure 3

Effect of BJO on apoptotic proteins. AML cells were treated with etoposide (10 μmol/L) and various concentrations of BJO as indicated for 6 hours. (a) The mRNA levels of apoptosis-related proteins were examined by RT-PCR as described in Materials and Methods. (b, c, and d) Western blotting analysis of procaspase-3, procaspase-9, Bcl-1, Mcl-1, Bax, Bid, pro-caspase-8, c-FLIP_(L/S), DR4, pro-DR5, Fas, XIAP and Survivin. Lane 1 and Lanes 3–5: cells were treated with or without BJO. Lane 2: cells were treated with etoposide (10 μmol/L). The level of each protein was determined using specific antibodies as described in Materials and Method.