Evidence-Based Complementary and Alternative Medicine

Research Article

Identification and Characterization of Cell Wall Proteins of a Toxic Dinoflagellate Alexandrium catenella Using 2-D DIGE and MALDI TOF-TOF Mass Spectrometry

Table 1

Functional categorization of CWPs from A. catenella DH01.


Spot no.	Accession no.	Identification of MS-blast	MS-blast score (HSPs)

Cell wall modifying enzymes

III	AACY01006738	Quinoprotein ethanol dehydrogenase	114 (2)
V	Q7S9I3	Acyl-CoA dehydrogenase	107 (2)
5	Q9F1X6	Phosphotransacetylase	100 (2)
8	Q8XTK4	Probable transmembrane protein	135 (3)
24	Q5WKD5	Mannonate dehydratase	104 (2)
27	Q635P5	Hydrolase, carbon-nitrogen family	59 (1)
37	Q7NLM8	Gll1094 protein	65 (1)
43	Q88D51	5,10-methylenetetrahydrofolate reductase	80 (1)
46	D00131	Tyrosinase	73 (1)
66	CP000025	8-amino-7-oxononanoate synthase	64 (1)
69	P51973	Competence protein comA	65 (1)
80	Q63QT9	Gamma-glutamyl phosphate reductase	107 (2)
81	Q5UU97	Enolase	141 (3)
98	Q9K7B3	BH3453 protein	102 (2)
102	Q72CF9	Methionine aminopeptidase	64 (1)

Transport/binding proteins and lipoproteins

I	Q39909	Luciferin-binding protein	110 (2)
17	O73697	Calcium channel alpha-1 subunit homolog	75 (1)
36	Q6FPN9	Similar to uniprotP40548 Saccharomyces cerevisiae YIL016w SNL1	76 (1)
85	Q926C3	Outer membrane lipoprotein omp16 homolog	97 (2)
97	CP000009	Carbamoyl-phosphate synthase large chain	70 (1)
100	Q833S0	ABC transporter, ATP-binding protein	97 (2)
112	Q6IV89	F1Fo-ATPase synthase f subunit	59 (1)

Signaling proteins

II	Q01369	Guanine nucleotide-binding protein beta subunit-like protein	152 (3)
18	Q7T0K6	Melanocortin 4 receptor	64 (1)
25	CAAJ01000020	Cg1 protein, putative	70 (1)
57	Q6WQQ4	Translocon-associated protein beta	67 (1)
72	AE006464	possible G-protein receptor	99 (2)
88	Q9VQM8	CG34393	68 (1)
103	Q98TY6	Tyrosine kinase negative regulator Cbl	137 (3)

Cell wall structure-related proteins

VI	Q8H6H9	Cell division inhibitor MinD	66 (1)
1	Q6N415	Putative D-alanyl-D-alanine ligase A	105 (2)
99	Q82D96	Putative UDP-glucose 4-epimerase	64 (1)
111	C2Q9T5	Penicillin-binding protein	66 (1)

Defense

IV	AE002181	polymorphic membrane protein B/C family	67 (1)
31	Q9WXP7	Dihydropteroate synthase	101 (2)
63	Q9Q6Y7	Vpu protein	65 (1)
74	Q8TR39	FmtA-like protein	70 (1)
109	Q9P3U2	SPAC328.04 protein	60 (1)

Uncharacterized proteins

32	Q9SKD8	At2g46420/F11C10.11	106 (2)
49	Q9W2X5	CG2962	60 (1)
15	Q89W76	hypothetical membrane protein	73 (1)
78	Q65173	PB407L	104 (2)