Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
Figure 13
EAS5-induced inhibition of oncogenic signaling pathways in NB4 cells. Western blot analysis for phosphorylated (p-) and total Akt (a), phosphorylated mTOR (b), phosphorylated and total ERK (c) of EAS5-treated NB4 cells. Phosphorylated Akt, mTOR and ERK were all dramatically inhibited by treatment with EAS5. Total protein level of Akt was also dramatically decreased by EAS5, while the total ERK protein was not changed. The cells were treated with PBS or EAS5 (3.75–30 μg/mL) for 72 h and then harvested. Cell lysates were then prepared for western blot analysis. EAS5: active fraction of THL.
