Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
Figure 8
THL-induced inhibition of oncogenic signaling pathways in NB4 cells. Western blot analysis for phosphorylated (p-) and total Akt (a), mTOR (b), Stat3 (c) and ERK (d) proteins of THL-treated NB4 cells. Phosphorylated Akt, mTOR, Stat3 and ERK were all significantly inhibited by treatment with THL. Total protein levels of Akt, mTOR and Stat3 were also significantly decreased by THL, while the total ERK protein was only slightly decreased by the highest dose of THL. The cells were treated with PBS or THL (0.375–3 mg/ml) for 72 h and then harvested. Cell lysates were then prepared for western blot analysis.
