Inhibitory Effect of Polyphenol-Rich Fraction from the Bark of Acacia mearnsii on Itching Associated with Allergic Dermatitis

Figure 3

AQP3 mRNA (a) and protein (b) expression levels in the skin. HR-1 mice were given free access to a normal diet (control group), HR-AD diet (AD group), HR-AD diet with 1.5% PrA, or HR-AD diet with 3.0% PrA for 6 weeks. (a) The skin was harvested, and the AQP3 mRNA expression level was measured using real-time RT-PCR. GAPDH was used as a housekeeping gene. (b) The skin was harvested, and the AQP3 protein expression level was measured using Western blotting. mRNA and protein expression levels are presented as the percentage of the control group (100%). Data represent the mean ± SD for 8 mice. Tukey’s test: ***𝑃<0.001 versus control group, ^#𝑃<0.05, ^##𝑃<0.01, and ^###𝑃<0.001 versus AD group.

(a)

(b)