Research Article

Involvement of Heme Oxygenase-1 Induction in the Cytoprotective and Immunomodulatory Activities of Viola patrinii in Murine Hippocampal and Microglia Cells

Figure 8

Effects of NNMBS275 on nuclear translocation of Nrf2 in BV2 cells and HT22. (a) BV2 cells and (b) HT22 cells were treated with 200 μg/mL of NNMBS275 for 0.5, 1, and 1.5 h. The nuclei were fractionated from the cytosol using PER-mammalian protein extraction buffer as described in Section 2. Nrf2 protein was detected by western blot analysis, and representative blots of 3 independent experiments are shown.
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(a) BV2 microglia
128019.fig.008b
(b) HT22 cells