Research Article

Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo

Figure 3

The effects of ethyl-acetate fractions from the stem of A. callosa var. callosa (EAAC) on lipopolysaccharide-(LPS-) induced TNF-α (a), iNOS, COX-2 (b), and HO-1 ((c) and (d)) expressions. TNF-α concentration in the medium was determined using ELISA kit. The protein expression of iNOS and COX-2 by cells were incubated for 24 h with 100 ng/mL of LPS in the absence or the presence of EAAC (0, 62.5, 125, and 250 μg/mL). The protein expression of HO-1 by cells were incubated for the indicated times or different concentration of EAAC (0, 62.5, 125, and 250 μg/mL) for 12 h. Lysed cells were then prepared and subjected to western blotting using an antibody specific for iNOS, COX-2, and HO-1. β-actin was used as an internal control. Representative western blot from two separate experiments is shown. Relative iNOS, COX-2, and HO-1 protein levels were calculated with reference to a LPS-stimulated culture. ###Compared with sample of control group. The data were presented as mean ± S.D. for three different experiments performed in triplicate. * , ** , and *** were compared with LPS-alone group.
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