Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo
Table 1
Radical scavenging activity of methanol extract of Actinidia callosa var. callosa (MAC) determined by TEAC, DPPH assay, and inhibition of NO production in LPS-induced RAW 264.7 macrophages by MAC and its reference compounds.*
Samples
TEAC (μg/mg extract)
DPPH scavenging activity EC50 (μg/mL)
Total phenolic (mg CE/g)a
Total flavonoid (mg RE/g)b
NO production IC50 value (μg/mL)
Methanol extract
90 ± 1.58
215.48 ± 3.59
299.92 ± 2.13
9.34 ± 0.41
237.24 ± 3.79
n-hexane fraction
14.26 ± 0.59
1754.29 ± 8.71
177.33 ± 1.49
8.29 ± 0.35
>250
Ethyl-acetate fraction
130.69 ± 2.16
132.69 ± 4.32
519.54 ± 2.47
18.67 ± 0.49
59.23 ± 2.17
n-butanol fraction
125.86 ± 1.47
135.31 ± 3.19
415.06 ± 1.47
6.22 ± 0.23
>250
Water fraction
98.43 ± 1.05
162.78 ± 1.15
361.45 ± 1.29
6.84 ± 0.51
>250
Protocatechuic acid
1552.14 ± 2.87
12.46 ± 1.57
(—)
(—)
>100
Catechin
1613.36 ± 4.53
9.45 ± 0.54
(—)
(—)
86.18 ± 0.15
Vanillic acid
532.75 ± 4.38
46.84 ± 2.62
(—)
(—)
>100
Caffeic acid
1837.28 ± 6.32
8.57 ± 0.54
(—)
(—)
32.26 ± 0.23
Syringic acid
1329.10 ± 5.37
10.25 ± 0.35
(—)
(—)
>100
Epicatechin
1257.56 ± 3.59
20.72 ± 1.23
(—)
(—)
>100
BHT
321.36 ± 2.56
103.45 ± 3.89
(—)
(—)
(—)
Indomethacin
(—)
(—)
(—)
(—)
74.36 ± 0.12
*Values represented mean ± S.D. of three parallel measurements ().
aData expressed in μg catechin equivalent/mg dry weight (mg CE/g).
bData expressed in μg rutin equivalent/mg dry weight (mg RE/g).
