Autophagy Inhibition Enhances Apoptosis Induced by Dioscin in Huh7 Cells

Figure 1

Dioscin exerts apoptotic effect on Huh7 cells. (a) Structure of dioscin. (b) Cell viability of Huh7 cells cultured in presence of dioscin for 24 and 48 hours, as analyzed by MTT assay. (c) Cell survival and cell death, determined by cell count, of Huh7 cell treated with dioscin for 24 hours. Cells were treated with an indicated concentration of dioscin for 24 hours and then subjected to DAPI staining (d) and JC-1 (e) followed by an observation under fluorescence microscopy. For quantitative analysis of apoptosis, dioscin-treated cells were harvested and then subjected to Annexin-V and PI double-stained flow cytometry (f). Results are shown as mean ± SD from 3 determinations per condition repeated 3 times. **𝑃<0.01; ***𝑃<0.001, compared with the control (0 μM); ^#𝑃<0.05; ^##𝑃<0.01; ^###𝑃<0.001, compared with the control (0 μM). Scale bars = 100 μm.

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