Autophagy Inhibition Enhances Apoptosis Induced by Dioscin in Huh7 Cells
Figure 3
Dioscin may enhance the activation of MAPK in Huh7 cells. Cells were treated with an indicated concentration of dioscin for 24 hours and then analyzed by western blotting with an appropriate antibody to investigate the expression of phosphorylation of ERK1/2, JNK1/2, and p38 with β-actin acting as an internal control (a). Cells were treated with 5 μM dioscin for 24 hours in the presence or absence of 10 μM U0126, 20 μM SB203580, and 20 μM SP600125 and then subjected to MTT assay for cell viability. Results are shown as mean ± SD from 3 determinations per condition repeated 3 times (b). Huh7 cells were treated with 5 μM dioscin for 24 hours in the presence or absence of 10 μM U0126 and then subjected to Annexin-V and PI double-stained flow cytometry (c). Results are shown as mean ± SD. ***, control versus dioscin; #, dioscin versus MAPK inhibitor plus dioscin; ##, dioscin versus U0126 plus dioscin.
