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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 134512, 11 pages
http://dx.doi.org/10.1155/2012/134512
Research Article

Autophagy Inhibition Enhances Apoptosis Induced by Dioscin in Huh7 Cells

1School of Medical Laboratory and Biotechnology, Chung Shan Medical University, 110, Section 1, Chien-Kuo N. Road, Taichung 402, Taiwan
2Institute of Medicine, Chung Shan Medical University, 110, Section 1, Chien-Kuo N. Road, Taichung 402, Taiwan
3Department of Medical Research, Chung Shan Medical University Hospital, 110, Section 1, Chien-Kuo N. Road, Taichung 402, Taiwan
4Department of Biochemistry and Institute of Biochemistry and Biotechnology, Chung Shan Medical University, 110, Section 1, Chien-Kuo N. Road, Taichung 402, Taiwan
5Department of Internal Medicine, Chung Shan Medical University Hospital, 110, Section 1, Chien-Kuo N. Road, Taichung 402, Taiwan
6Department of Internal Medicine, School of Medicine, Chung Shan Medical University, 110, Section 1, Chien-Kuo N. Road, Taichung 402, Taiwan
7Department of Clinical Laboratory, Chung Shan Medical University Hospital, 110, Section 1, Chien-Kuo N. Road, Taichung 402, Taiwan

Received 9 August 2012; Accepted 2 October 2012

Academic Editor: William CS Cho

Copyright © 2012 Ming-Ju Hsieh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. V. Ivanov, M. W. Roomi, T. Kalinovsky, A. Niedzwiecki, and M. Rath, “Anti-atherogenic effects of a mixture of ascorbic acid, lysine, proline, arginine, cysteine, and green tea phenolics in human aortic smooth muscle cells,” Journal of Cardiovascular Pharmacology, vol. 49, no. 3, pp. 140–145, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. A. B. da Rocha, R. M. Lopes, and G. Schwartsmann, “Natural products in anticancer therapy,” Current Opinion in Pharmacology, vol. 1, no. 4, pp. 364–369, 2001. View at Publisher · View at Google Scholar · View at Scopus
  3. C. W. Chua and S. P. Choo, “Targeted therapy in hepatocellular carcinoma,” International Journal of Hepatology, vol. 2011, Article ID 348297, 11 pages, 2011. View at Publisher · View at Google Scholar
  4. M. L. Ho, Y. S. Hsieh, J. Y. Chen et al., “Antimetastatic potentials of Dioscorea nipponica on melanoma in vitro and in vivo,” Evidence-Based Complementary and Alternative Medicine, vol. 2011, Article ID 507920, 13 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  5. S. H. Du, W. Y. Liu, T. J. Fu, B. G. Li, and C. D. Xia, “Isolation and identification of steroidal saponins in total saponin from Dioscorea nipponica Makino,” Yaoxue Xuebao, vol. 37, no. 4, pp. 267–270, 2002. View at Scopus
  6. Y. Zhang, H. Z. Li, Y. J. Zhang et al., “Atropurosides A-G, new steroidal saponins from Smilacina atropurpurea,” Steroids, vol. 71, no. 8, pp. 712–719, 2006. View at Publisher · View at Google Scholar · View at Scopus
  7. V. T. B. Nguyen, N. Darbour, C. Bayet et al., “Selective modulation of P-glycoprotein activity by steroidal saponines from Paris polyphylla,” Fitoterapia, vol. 80, no. 1, pp. 39–42, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. T. Wang, Z. Liu, J. Li et al., “Determination of protodioscin in rat plasma by liquid chromatography-tandem mass spectrometry,” Journal of Chromatography B, vol. 848, no. 2, pp. 363–368, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. Y. Wang, H. C. Yim, Z. Yang, J. F. Chiu, C. M. Che, and Q. Y. He, “Proteomic approach to study the cytotoxicity of dioscin (saponin),” Proteomics, vol. 6, no. 8, pp. 2422–2432, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. J. Cai, M. Liu, Z. Wang, and Y. Ju, “Apoptosis induced by Dioscin in Hela cells,” Biological and Pharmaceutical Bulletin, vol. 25, no. 2, pp. 193–196, 2002. View at Publisher · View at Google Scholar · View at Scopus
  11. Y. Wang, C. M. Che, J. F. Chiu, and Q. Y. He, “Dioscin (saponin)-induced generation of reactive oxygen species through mitochondria dysfunction: a proteomic-based study,” Journal of Proteome Research, vol. 6, no. 12, pp. 4703–4710, 2007. View at Publisher · View at Google Scholar · View at Scopus
  12. B. T. Sun, L. H. Zheng, Y. L. Bao et al., “Reversal effect of Dioscin on multidrug resistance in human hepatoma HepG2/adriamycin cells,” European Journal of Pharmacology, vol. 654, no. 2, pp. 129–134, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. Z. Liu and M. J. Lenardo, “Reactive oxygen species regulate autophagy through redox-sensitive proteases,” Developmental Cell, vol. 12, no. 4, pp. 484–485, 2007. View at Publisher · View at Google Scholar · View at Scopus
  14. D. Gozuacik and A. Kimchi, “Autophagy and cell death,” Current Topics in Developmental Biology, vol. 78, pp. 217–245, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. L. Yu, A. Alva, H. Su et al., “Regulation of an ATG7-beclin 1 program of autophaglic cell death by caspase-8,” Science, vol. 304, no. 5676, pp. 1500–1502, 2004. View at Publisher · View at Google Scholar · View at Scopus
  16. T. Kanzawa, Y. Kondo, H. Ito, S. Kondo, and I. Germano, “Induction of autophagic cell death in malignant glioma cells by arsenic trioxide,” Cancer Research, vol. 63, no. 9, pp. 2103–2108, 2003. View at Scopus
  17. C. P. Chang, M. C. Yang, H. S. Liu, Y. S. Lin, and H. Y. Lei, “Concanavalin A induces autophagy in hepatoma cells and has a therapeutic effect in a murine in situ hepatoma model,” Hepatology, vol. 45, no. 2, pp. 286–296, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. M. Górka, W. M. Daniewski, B. Gajkowska, E. Łusakowska, M. M. Godlewski, and T. Motyl, “Autophagy is the dominant type of programmed cell death in breast cancer MCF-7 cells exposed to AGS 115 and EFDAC, new sesquiterpene analogs of paclitaxel,” Anti-Cancer Drugs, vol. 16, no. 7, pp. 777–788, 2005. View at Publisher · View at Google Scholar · View at Scopus
  19. A. Kelekar, “Autophagy,” Annals of the New York Academy of Sciences, vol. 1066, pp. 259–271, 2005. View at Publisher · View at Google Scholar · View at Scopus
  20. I. V. Smirnova, D. C. Bittel, R. Ravindra, H. Jiang, and G. K. Andrews, “Zinc and cadmium can promote rapid nuclear translocation of metal response element-binding transcription factor-1,” The Journal of Biological Chemistry, vol. 275, no. 13, pp. 9377–9384, 2000. View at Publisher · View at Google Scholar · View at Scopus
  21. Y. R. Lee, H. Y. Lei, M. T. Liu et al., “Autophagic machinery activated by dengue virus enhances virus replication,” Virology, vol. 374, no. 2, pp. 240–248, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. L. Casciola-Rosen, A. Rosen, M. Petri, and M. Schlissel, “Surface blebs on apoptotic cells are sites of enhanced procoagulant activity: implications for coagulation events and antigenic spread in systemic lupus erythematosus,” Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 4, pp. 1624–1629, 1996. View at Publisher · View at Google Scholar · View at Scopus
  23. C. Dong, R. J. Davis, and R. A. Flavell, “MAP kinases in the immune response,” Annual Review of Immunology, vol. 20, pp. 55–72, 2002. View at Publisher · View at Google Scholar · View at Scopus
  24. J. Matsukawa, A. Matsuzawa, K. Takeda, and H. Ichijo, “The ASK1-MAP kinase cascades in mammalian stress response,” Journal of Biochemistry, vol. 136, no. 3, pp. 261–265, 2004. View at Publisher · View at Google Scholar · View at Scopus
  25. C. Kitanaka and Y. Kuchino, “Caspase-independent programmed cell death with necrotic morphology,” Cell Death and Differentiation, vol. 6, no. 6, pp. 508–515, 1999. View at Scopus
  26. H. W. Yim, H. S. Jong, T. Y. Kim et al., “Cyclooxygenase-2 inhibits novel ginseng metabolite-mediated apoptosis,” Cancer Research, vol. 65, no. 5, pp. 1952–1960, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. T. Shintani and D. J. Klionsky, “Autophagy in health and disease: a double-edged sword,” Science, vol. 306, no. 5698, pp. 990–995, 2004. View at Publisher · View at Google Scholar · View at Scopus
  28. Y. Kondo, T. Kanzawa, R. Sawaya, and S. Kondo, “The role of autophagy in cancer development and response to therapy,” Nature Reviews Cancer, vol. 5, no. 9, pp. 726–734, 2005. View at Publisher · View at Google Scholar · View at Scopus
  29. M. Høyer-Hansen and M. Jäättelä, “Autophagy: an emerging target for cancer therapy,” Autophagy, vol. 4, no. 5, pp. 574–580, 2008. View at Scopus
  30. M. T. Rosenfeldt and K. M. Ryan, “The role of autophagy in tumour development and cancer therapy,” Expert Reviews in Molecular Medicine, vol. 11, article e36, 2009. View at Scopus
  31. A. Herman-Antosiewicz, D. E. Johnson, and S. V. Singh, “Sulforaphane causes autophagy to inhibit release of cytochrome c and apoptosis in human prostate cancer cells,” Cancer Research, vol. 66, no. 11, pp. 5828–5835, 2006. View at Publisher · View at Google Scholar · View at Scopus
  32. H. Ko, Y. J. Kim, J. S. Park, J. H. Park, and H. O. Yang, “Autophagy inhibition enhances apoptosis induced by ginsenoside Rkl in hepatocellular carcinoma cells,” Bioscience, Biotechnology and Biochemistry, vol. 73, no. 10, pp. 2183–2189, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. L. Longo, F. Platini, A. Scardino, O. Alabiso, G. Vasapollo, and L. Tessitore, “Autophagy inhibition enhances anthocyanin-induced apoptosis in hepatocellular carcinoma,” Molecular Cancer Therapeutics, vol. 7, no. 8, pp. 2476–2485, 2008. View at Publisher · View at Google Scholar · View at Scopus
  34. T. Kanzawa, I. M. Germano, T. Komata, H. Ito, Y. Kondo, and S. Kondo, “Role of autophagy in temozolomide-induced cytotoxicity for malignant glioma cells,” Cell Death and Differentiation, vol. 11, no. 4, pp. 448–457, 2004. View at Publisher · View at Google Scholar · View at Scopus
  35. M. C. Maiuri, L. Galluzzi, E. Morselli, O. Kepp, S. A. Malik, and G. Kroemer, “Autophagy regulation by p53,” Current Opinion in Cell Biology, vol. 22, no. 2, pp. 181–185, 2010. View at Publisher · View at Google Scholar · View at Scopus
  36. J. F. Lin, Y. C. Lin, Y. H. Lin et al., “Zoledronic acid induces autophagic cell death in human prostate cancer cells,” Journal of Urology, vol. 185, no. 4, pp. 1490–1496, 2011. View at Publisher · View at Google Scholar · View at Scopus
  37. E. Tasdemir, M. C. Maiuri, L. Galluzzi et al., “Regulation of autophagy by cytoplasmic p53,” Nature Cell Biology, vol. 10, no. 6, pp. 676–687, 2008. View at Publisher · View at Google Scholar · View at Scopus
  38. D. Crighton, S. Wilkinson, J. O'Prey et al., “DRAM, a p53-induced modulator of autophagy, is critical for apoptosis,” Cell, vol. 126, no. 1, pp. 121–134, 2006. View at Publisher · View at Google Scholar · View at Scopus
  39. Z. Feng, H. Zhang, A. J. Levine, and S. Jin, “The coordinate regulation of the p53 and mTOR pathways in cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 23, pp. 8204–8209, 2005. View at Publisher · View at Google Scholar · View at Scopus
  40. I. K. Choi, Y. S. Cho, H. J. Jung, and H. J. Kwon, “Autophagonizer, a novel synthetic small molecule, induces autophagic cell death,” Biochemical and Biophysical Research Communications, vol. 393, no. 4, pp. 849–854, 2010. View at Publisher · View at Google Scholar · View at Scopus
  41. J. Y. U. Hung, Y. A. L. Hsu, C. T. Li et al., “6-shogaol, an active constituent of dietary ginger, induces autophagy by inhibiting the AKT/mTOR pathway in human non-small cell lung cancer A549 cells,” Journal of Agricultural and Food Chemistry, vol. 57, no. 20, pp. 9809–9816, 2009. View at Publisher · View at Google Scholar · View at Scopus
  42. J. C. Reed, “Apoptosis-targeted therapies for cancer,” Cancer Cell, vol. 3, no. 1, pp. 17–22, 2003. View at Publisher · View at Google Scholar · View at Scopus
  43. J. Yu, S. Tiwari, P. Steiner, and L. Zhang, “Differential apoptotic response to the proteasome inhibitor bortezomib (VELCADE, PS-341) in bax-deficient and p21-deficient colon cancer cells,” Cancer Biology and Therapy, vol. 2, no. 6, pp. 694–699, 2003. View at Publisher · View at Google Scholar · View at Scopus