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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 138954, 11 pages
http://dx.doi.org/10.1155/2012/138954
Research Article

Analgesic and Anti-Inflammatory Activities of the Ethanolic Extract of Artemisia morrisonensis Hayata in Mice

1Graduate Institute of Ecology and Evolutionary Biology, College of Life Sciences, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
2Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
3Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
4Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan
5Department of Optometry, Jen-Teh Junior College of Medicine, Nursing and Management, No. 79-9 Sha-Luen-Hu, Xi Zhou Li, Houloung 35664, Taiwan
6School of Pharmacy, College of Pharmacy, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
7Department of Forestry, College of Agriculture and Natural Resources, National Chung Hsing University, No. 250 Kuo-Kuang Road, Taichung 40227, Taiwan

Received 15 June 2012; Accepted 8 December 2012

Academic Editor: Annie Shirwaikar

Copyright © 2012 Shen-Chieh Chou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate the possible analgesic and anti-inflammatory mechanisms of the ethanolic extract of A. morrisonensis Hayata ( ). Two models were employed for evaluation of the analgesic effects: acetic acid-induced writhing response and formalin-induced paw licking. The results demonstrated that decreased writhing response for both the acetic acid assay and the licking time in the formalin test. The anti-inflammatory effect was evaluated by paw edema of mice induced by λ-carrageenan. significantly decreased induced paw edema three to four hours after λ-carrageenan injection. Additionally, the results indicated that the anti-inflammatory mechanism of may be due to the declined levels of nitric oxide (NO) and malondialdehyde (MDA) in the edematous paw. Furthermore, decreased the tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) levels, leading to the reduction of prostaglandins and subsequently alleviated edema. Isolation and purification of the extract determined p-hydroxyacetophenone to be a major component at 130 mg/g of extract. No mortality was observed in the acute toxicity test given at the dose of 10 g/kg. This study demonstrated the possible mechanisms for the analgesic and anti-inflammatory effects of for mice and provided evidence for the ethnobotanical uses of A. morrisonensis in treating inflammatory diseases.