Research Article

Bioassay-Guided Isolation and HPLC Determination of Bioactive Compound That Relate to the Antiplatelet Activity (Adhesion, Secretion, and Aggregation) from Solanum lycopersicum

Table 1

Effect of extracts, fraction, and antiplatelet compound on platelet aggregation.

ADP
Maximum aggregation (%)SlopeArea under the curve Lag time (s)

Total extract (1 mg/mL)51 ± 8*38 ± 2*206 ± 10*24 ± 3
Aqueous extract (1 mg/mL)37 ± 2*53 ± 7*140 ± 56*38 ± 5
Subfraction B (1 mg/mL)1 ± 8*6 ± 6*2 ± 3*>120*
Adenosine
 2.3 μM56 ± 2*58 ± 2*261 ± 9*27 ± 1
 4.6 μM40 ± 5*44 ± 6*201 ± 14*34 ± 1
 43 μM26 ± 8*18 ± 12*123 ± 15*20 ± 1
 457 μM21 ± 6*28 ± 8*98 ± 11*53 ± 2*
Negative control 85 ± 2 104 ± 14 393 ± 21 30 ± 1

Values were presented in mean ± SD ( ). Band C corresponds to adenosine.
ADP 8 μM. * versus negative control (saline 0.9%).