Bioassay-Guided Isolation and HPLC Determination of Bioactive Compound That Relate to the Antiplatelet Activity (Adhesion, Secretion, and Aggregation) from Solanum lycopersicum
Table 1
Effect of extracts, fraction, and antiplatelet compound on platelet aggregation.
ADP
Maximum aggregation (%)
Slope
Area under the curve
Lag time (s)
Total extract (1 mg/mL)
51 ± 8*
38 ± 2*
206 ± 10*
24 ± 3
Aqueous extract (1 mg/mL)
37 ± 2*
53 ± 7*
140 ± 56*
38 ± 5
Subfraction B (1 mg/mL)
1 ± 8*
6 ± 6*
2 ± 3*
>120*
Adenosine
2.3 μM
56 ± 2*
58 ± 2*
261 ± 9*
27 ± 1
4.6 μM
40 ± 5*
44 ± 6*
201 ± 14*
34 ± 1
43 μM
26 ± 8*
18 ± 12*
123 ± 15*
20 ± 1
457 μM
21 ± 6*
28 ± 8*
98 ± 11*
53 ± 2*
Negative control
85 ± 2
104 ± 14
393 ± 21
30 ± 1
Values were presented in mean ± SD (). Band C corresponds to adenosine. ADP 8 μM. * versus negative control (saline 0.9%).