A Standardized Chinese Herbal Decoction, Kai-Xin-San, Restores Decreased Levels of Neurotransmitters and Neurotrophic Factors in the Brain of Chronic Stress-Induced Depressive Rats

Table 1

Primer sequences, length of PCR products and optimal annealing temperature for each gene used in real-time quantitative PCR.

Primer	Sequence (5′-3′)	Source	bp	Ta; (°C)
TH-S	CCA GTT CTC CCA GGA CAT TGG AC	NM_012740.3	312	59
TH-AS	GAG GCA TAG TTC CTG AGC TTG TCC
DBH-S	GAA GAA TGC TGT GAC TGT CCA CCA G	NM_013158.2	387	59
DBH-AS	CAG AGG CTG CAG GTT CCA GTT AC
AADC-S	GTT GTC ACC CTA GGA ACC ACA TCT TG	NM_012545.3	444	59
AADC-AS	CTC ATG AGA CAG CTT CAC GTG CTT TC
MAOA-S	GCC AAA GTT CTG GGA TCT CAA GAA GC	NM_033653.1	204	59
MAOA-AS	CAC CAG TGA TCT TGA GCA GAC CAG
MAOB-S	GAG AAG AAC TGG TGT GAG GAG CAG	NM_013198.1	342	59
MAOB-AS	AGC TGT TGC TGA CAA GAT GGT GGT
COMT-S	GGT GAC GCG AAA GGC CAA ATC ATG	NM_012531.2	351	59
COMT-AS	CAG GCC ACA TTT CTC CAG GAG AAG
DAT-S	GGT TCT ACG GCG TCC AGC AAT TC	M80570.1	291	59
DAT-AS	CAT AGG CCA GTT TCT CCC GGA AG
VMAT2-S	GGT GGA CTC CTC TAT GAT GCC TAT C	NM_013031.3	351	59
VMAT2-AS	CTC CTT AGC AGG TGG ACT TCG AAG
NET-S	CAG GTT CAG CAA TGA CAT CCA GCA G	NM_031343.1	282	59
NET-AS	GTG ATT CCG TAG GCC ACT CTC TC
DrD2-S	AAC TGT ACC CAC CCT GAG GAC ATG	NM_012547.1	236	59
DrD2-AS	CTG TCA GGG TTG CTA TGT AGG CC
Adra1A-S	TGG TGG GTT GCT TCG TCC TCT G	NM_017191.2	211	59
Adra1A-AS	CGA AGA CAC TGG ATT CGC AGG AC
TPH-S	CAC CCA GGA TTC AAG GAC AAC GTC	NM_173839.2	421	59
TPH-AS	CAC TGT GAA GCC AGA TCG CTC TTT C
SERT-S	ATG GTT CGT GCT CAT CGT GGT CAT C	NM_013034.3	268	59
SERT-AS	GAT GAA CAG GAG AAA CAG AGG GCT G
Htr1A-S	CAT CAG CAA GGA CCA CGG CTA C	NM_012585.1	353	59
Htr1A-AS	GGA AGG TGC TCT TTG GAG TTG CC
NGF-S	CAC TCT GAG GTG CAT AGC GTA ATG TC	XP_001067130.2	374	59
NGF-AS	CTG TGA GTC CTG TTG AAG GAG ATT GTA C
BDNF-S	GAG CTG AGC GTG TGT GAC AGT ATT AG	BC087634	229	59
BDNF-AS	ATT GGG TAGT TCG GCA TTG CGA GTT C
GDNF-S	GCG CTG ACC AGT GAC TCC AAT ATG	AF497634	318	59
GDNF-AS	CGC TTC ACA GGA ACC GCT ACA ATAT C
NT3-S	ACA AGC TCT CCA AGC AGA TGG TAG ATG	M61179.1	310	59
NT3-AS	TCT CCT CGG TGA CTC TTA TGC TCT G
NT4-S	TCA GTA CTT CTT CGA GAC GCG CTG	NM_013184.3	135	59
NT4-AS	GGC ACA TAG GAC TGT TTA GCC TTG CAT
NT5-S	ATG CAG TGA GTG GCT GGG TGA C	S69323.1	229	59
NT5-AS	GTT TAG CCT TGC ATT CTG AGA GCC AG
TrkA-S	ACC TCA ACC GTT TCC TCC GGT C	M85214.	330	57
TrkA-AS	CTC GAT CGC CTC AGT GTT GGA GA
TrkB-S	CGG GAG CAT CTC TCG GTC TAT G	M55291.1	221	57
TrkB-AS	CAA ATG TGT CCG GCT TGA GCT GG
TrkC-S	CAC TGT CTA CTA CCC TCC ACG TG	L03813.1	253	57
TrkC-AS	CTC TCT GGA AAG GGC TCC TTA AGG
GAPDH-S	AAC GGA TTT GGC CGT ATT GG	Lee et al., 2009 [10]	516	57
GAPDH-AS	CTT CCC GTT CAG CTC TGG G

Abbreviations: S: sense primer; AS: antisense primer; TH: tyrosine hydroxylase; DBH: dopamine β-hydroxylase; AADC: aromatic acid decarboxylase; MAO_A: monoamine oxidase A; MAO_B: monoamine oxidase B; COMT: catechol-O-methyltransferase; DAT: dopamine transporter; VMAT2: vesicular monoamine transporter 2; NET: norepinepherine transporter; DrD2: dopamine receptor D2; Adra1A: adrenergic receptor α1A; Htr 1A: serotonin receptor 1A; TPH: tryptophan hydroxylase; SERT: serotonin transporter; Htr1a: serotonin receptor 1A; NGF: nerve growth factor; BDNF: brain derived neurotrophic factor; GDNF: glial-cell-line-derived neurotrophic factor; NT3: neurotrophin 3; NT4: neurotrophin 4; NT5: neurotrophin 5; Trk A: tyrosine kinase receptor A; Trk B: tyrosine kinase receptor B; Trk C: tyrosine kinase receptor C; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.