Figure 3: Effects of Sal B and losartan on Ang II-induced HSC proliferation. (a) HSC-T6 or (b) primary HSC cells were seeded in 96-well plates in DMEM medium with 10% or 20% FBS, respectively. After 24 h, cells were pretreated with Sal B (10−5 M), losartan (10−6 M), or vehicle in DMEM medium with 0.5% FBS for 4 h, and then incubated with Ang II (10−6 M) for another 24 h. Cells proliferation was determined by MTT method. Each bar represents the mean ± S.D. ** versus normal; ## versus Ang II alone group.