160726.fig.004
Figure 4: Effects of Sal B and losartan on Ang II-induced α-SMA and Col-I expression in HSC. HSC-T6 cells or primary rat HSC were seeded in 6 cm dishes in DMEM medium with 10% FBS. After 24 h, cells were pretreated with Sal B (10−5 M), losartan (10−6 M), or vehicle in DMEM medium with 0.5% FBS for 4 h, and then incubated with Ang II (10−6 M) for another 24 h. Western blot and real-time RT-PCR were performed to detect α-SMA and Col-I level. (a) α-SMA expression in HSC-T6 cells; (b) quantities for densitometric analysis of α-SMA; (c) Col-I expression in HSC-T6 cells; (d) quantities for densitometric analysis of Col-I; (e) Col-I mRNA levels in HSC-T6 cells; (f) Col-I mRNA levels in primary rat HSC; (g) staining for F-actin in HSC-T6 cells; (h) staining for α-SMA expression in primary rat HSC. Each bar represents the mean ± S.D. of three independent experiments. ** versus normal; ## versus Ang II alone group.