160726.fig.006
Figure 6: Effects of Sal B and losartan on Ang II-induced ERK and c-Jun phosphorylation and AT1R expression in HSC. HSC-T6 cells or primary rat HSC were seeded in 6 cm dishes in DMEM medium with 10% FBS. After 24 h, cells were pretreated with Sal B (10−5 M), losartan (10−6 M) or vehicle in DMEM medium with 0.5% FBS for 4 h, then incubated with Ang II (10−6 M) for indicated times. (a) Western blot analysis of total and phosphorylated ERK in HSC-T6 cells; (b) quantities for densitometric analysis of ERK and pERK; (c) western blot analysis of c-Jun and p-c-Jun in HSC-T6 cells; (d) quantities for densitometric analysis of c-Jun and p-c-Jun; (e) and (g) western blot analysis of AT1R protein level in HSC-T6 cells and primary rat HSC, respectively; (f) and (h) quantities for densitometric analysis of AT1R in HSC-T6 cells and primary rat HSC, respectively. Each bar represents the mean ± S.D. of three independent experiments. ** versus normal; ## versus Ang II alone group.