Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo
Table 2
Matrix metalloproteinase-1 levels: inhibitory effect of A. scholaris extract in UVB-irradiated human primary fibroblasts. Effect of all-trans retinoic acid or retinol cotreatment on matrix metalloproteinase-1 expression inhibitory activity of A. scholaris extract in UVB-irradiated human primary fibroblasts.
(a)
Test conditions
ng/mL
% of inhibition
ASE 0.1 ppm + UVB 40 mJ
621.8 ± 27.8**
59.4
ASE 1 ppm + UVB 40 mJ
451.0 ± 38.4**
98.8
ASE 10 ppm + UVB 40 mJ
331.2 ± 22.2**
126.5
ATRA 1 M + UVB 40 mJ
557.4 ± 9.7**
74.3
ATRA 5 M + UVB 40 mJ
505.3 ± 64.1*
86.3
ROL 1 M + UVB 40 mJ
708.9 ± 30.8*
39.2
ROL 5 M + UVB 40 mJ
574.9 ± 51.1*
70.2
UVB 40 mJ
878.5 ± 30.2
0
Control
446.0 ± 28.5
100.0
MMP-1: Matrix metalloproteinase-1; ASE: Alstonia scholaris extract; ATRA: all-trans retinoic acid; ROL: retinol. * and ** compared with UVB-irradiated group values.
(b)
Test substance
ng/mL
% of inhibition
ATRA 1 M + UVB 40 mJ
557.4 ± 9.7**
74.3
ASE 0.1 ppm + ATRA 1 M + UVB 40 mJ
424.6 ± 6.9**
105.0
ASE 1 ppm + ATRA 1 M + UVB 40 mJ
379.7 ± 18.3**
115.3
ASE 10 ppm + ATRA 1 M + UVB 40 mJ
335.6 ± 50.5**
125.5
ROL 1 M + UVB 40 mJ
708.9 ± 30.8*
39.2
ASE 0.1 ppm + ROL 1 M + UVB 40 mJ
522.5 ± 13.3**
82.3
ASE 1 ppm + ROL 1 M + UVB 40 mJ
439.4 ± 38.4**
101.5
ASE 10 ppm + ROL 1 M + UVB 40 mJ
389.3 ± 28.1**
113.1
UVB 40 mJ
878.5 ± 30.2
0.0
Control
446.0 ± 28.5
100.0
MMP-1: Matrix metalloproteinase-1; ASE: Alstonia scholaris extract; ATRA: all-trans retinoic acid; ROL: retinol. * and ** compared with UVB-irradiated group values.
