Sesamin: A Naturally Occurring Lignan Inhibits CYP3A4 by Antagonizing the Pregnane X Receptor Activation
Figure 5
Transactivation of the CYP3A4 promoter by human CAR and rat PXR in the presence of sesamin and/or rifampin. HepG2 cells were cotransfected with a vector control (pcDNA3) and (a) human CAR expression plasmids (pcDNA3-CAR) or (b) rat PXR expression plasmids (pcDNA3-rPXR), a CYP3A4 luciferase reporter plasmid, and pRC-CMV-β-galactosidase vectors. Subsequently, the transfected cells were exposed to sesamin and/or rifampin for 24 h. The results are expressed as mean ± SD (); * and ***, as compared to rifampin-treated cells.
