256561.fig.003a
(a)
256561.fig.003b
(b)
Figure 3: GP-derived reduction of DMN-induced α-SMA expression in livers and HSCs. Liver samples were excised 6 weeks after the first DMN injection. (a) The expression and localization of α-SMA in the liver were detected by immunohistochemical staining using a monoclonal antibody for α-SMA. Representative photomicrographs from controls, DMN treatment alone, DMN − Silymarin treatment, and DMN − GP treatment are shown. The original magnification was 100×. (b) Immunocytochemical staining of α-SMA and collagen I expression in cultured HSC. Cells were treated with or without 0.5 mg/mL GP extract for indicated time points and stained with FITC-conjugated α-SMA and collagen I antibodies. Representative photomicrographs from controls, DMN treatment alone, DMN − Silymarin treatment, and DMN − GP treatment are shown. The original magnification was 400×.