Research Article

Ginseng and Its Active Components Ginsenosides Inhibit Adipogenesis in 3T3-L1 Cells by Regulating MMP-2 and MMP-9

Table 1

Sequences of primers used for the RT-PCR assays.

GeneGene bankPrimer sequenceSize (bp)

MMP-2U65656Forward: 5′-atctggtgtctcccttacgg-3′ 
Reverse: 5′-gttgtcggacatcactgcac-3′
704
MMP-9AK161176Forward: 5′-tgcgaccacatcgaacttcg-3′  
Reverse: 5′-ccaagagggttttcttcttctctgg-3′
687
TIMP-1NM_001044384Forward: 5′-ggcatcctcttgttgctatcactg-3′  
Reverse: 5′-gtcatcttgatctcataacgctgg-3′
170
TIMP-2NM011594Forward: 5′-gagatcaagcagataaagatg-3′  
Reverse: 5′-gcctctggatggactgggtc-3′
320
PPAR NM_013124Forward: 5′-attctggcccaccaacttcgg-3′  
Reverse: 5′-tggaagcctgatgctttatcccca-3′
338
aP2K02109Forward: 5′-caaaatgtgtgatgcctttgtg-3′  
Reverse: 5′-ctcttcctttggctcatgcc-3′
415
LeptinNM_008493Forward: 5′-cctgctccagcagctgcaag-3′  
Reverse: 5′-ctatctgcagcacattttggga-3′
342
C/EBP NM_007678Forward: 5′-agacatcagcgcctacatcg-3′  
Reverse: 5′-gaccaccatgcacctgca-3′
416
NF-κB (p65)AK089489Forward: 5′-ccatcagggcagatctcaaacc-3′  
Reverse: 5′-gctgctgaaactctgagttgtc-3′
322
Sp1AB077988Forward: 5′-agtgcagcaggatggttctg-3′  
Reverse: 5′-taaagttcataattcccatg-3′
440
c-JunAK135023Forward: 5′-aactcggaccttctcacgtcg-3′  
Reverse: 5′-tgctgaggttggcgtagacc-3′
355
c-FosNM_010234Forward: 5′-cgttgcagactgagattgcc-3′  
Reverse: 5′-accggacaggtccacatctg-3′
356
-ActinJ00691Forward: 5′-tggaatcctgtggcatccatgaaa-3′  
Reverse: 5′-taaaacgcagctcagtaacagtcc-3′
350