Research Article
Ginseng and Its Active Components Ginsenosides Inhibit Adipogenesis in 3T3-L1 Cells by Regulating MMP-2 and MMP-9
Table 1
Sequences of primers used for the RT-PCR assays.
| Gene | Gene bank | Primer sequence | Size (bp) |
| MMP-2 | U65656 | Forward: 5′-atctggtgtctcccttacgg-3′ Reverse: 5′-gttgtcggacatcactgcac-3′ | 704 | MMP-9 | AK161176 | Forward: 5′-tgcgaccacatcgaacttcg-3′ Reverse: 5′-ccaagagggttttcttcttctctgg-3′ | 687 | TIMP-1 | NM_001044384 | Forward: 5′-ggcatcctcttgttgctatcactg-3′ Reverse: 5′-gtcatcttgatctcataacgctgg-3′ | 170 | TIMP-2 | NM011594 | Forward: 5′-gagatcaagcagataaagatg-3′ Reverse: 5′-gcctctggatggactgggtc-3′ | 320 | PPAR | NM_013124 | Forward: 5′-attctggcccaccaacttcgg-3′ Reverse: 5′-tggaagcctgatgctttatcccca-3′ | 338 | aP2 | K02109 | Forward: 5′-caaaatgtgtgatgcctttgtg-3′ Reverse: 5′-ctcttcctttggctcatgcc-3′ | 415 | Leptin | NM_008493 | Forward: 5′-cctgctccagcagctgcaag-3′ Reverse: 5′-ctatctgcagcacattttggga-3′ | 342 | C/EBP | NM_007678 | Forward: 5′-agacatcagcgcctacatcg-3′ Reverse: 5′-gaccaccatgcacctgca-3′ | 416 | NF-κB (p65) | AK089489 | Forward: 5′-ccatcagggcagatctcaaacc-3′ Reverse: 5′-gctgctgaaactctgagttgtc-3′ | 322 | Sp1 | AB077988 | Forward: 5′-agtgcagcaggatggttctg-3′ Reverse: 5′-taaagttcataattcccatg-3′ | 440 | c-Jun | AK135023 | Forward: 5′-aactcggaccttctcacgtcg-3′ Reverse: 5′-tgctgaggttggcgtagacc-3′ | 355 | c-Fos | NM_010234 | Forward: 5′-cgttgcagactgagattgcc-3′ Reverse: 5′-accggacaggtccacatctg-3′ | 356 | -Actin | J00691 | Forward: 5′-tggaatcctgtggcatccatgaaa-3′ Reverse: 5′-taaaacgcagctcagtaacagtcc-3′ | 350 |
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