Research Article

Isorhamnetin, A Flavonol Aglycone from Ginkgo biloba L., Induces Neuronal Differentiation of Cultured PC12 Cells: Potentiating the Effect of Nerve Growth Factor

Figure 3

NGF induces the neurite outgrowth in a dose-dependent manner. Cultured PC12 cultures were treated with NGF (0.3 to 50 ng/mL) for 72 hours. Cells were fixed with ice-cold 4% paraformaldehyde. The % of differentiated cell (upper panel) and length of neurite (lower panel) were counted as described in the Method section. Values are expressed as % of total cells in 100 counted cells, Mean SEM, . ** compared to the control.
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