Isorhamnetin, A Flavonol Aglycone from Ginkgo biloba L., Induces Neuronal Differentiation of Cultured PC12 Cells: Potentiating the Effect of Nerve Growth Factor
Figure 6
The potentiating effect of isorhamnetin is not mediated by NGF-induced signaling cascade. Cultured PC12 cells, serum starvation for 5 hours, were treated with NGF (5 ng/mL), isorhamnetin (Iso; 10 μM), and NGF (5 ng/mL) + isorhamnetin (Iso; 10 μM) for different time. Total TrkA and phosphorylated TrkA (a) total Akt and phosphorylated Akt (b) total Erk1/2 and phosphorylated Erk1/2 (c) were revealed by using specific antibodies. (d) Quantification plot of the phosphorylation level in treatment of 5 min was shown. Values are expressed as the fold of change (×Basal) against the control (no treatment; set as 1), and in mean SEM, . Representative images were shown. ** where compared to the control.
