Comprehensive Evaluation of Anti-hyperglycemic Activity of Fractionated Momordica charantia Seed Extract in Alloxan-Induced Diabetic Rats
Figure 3
SDS-PAGE analysis of different fractions of M. charantia seed extract under reducing condition. (a) Crude extract and subsequent fractions of M. charantia seed extract were electrophoresed on 15% SDS-PAGE stained with Coomassie brilliant blue CBB-R250. M indicates protein molecular weight markers in kilodaltons. The gel was stained with CBB-R250. (b) Identical gel of different M. charantia fractions as shown in panel (a) stained with silver stain. M indicates protein molecular weight markers. Lanes 1–5 represent parent fractions Crude M. charantia extract (Mc-C), insoluble fraction Mc-0, soluble fraction Mc-1 and fractions Mc-2 and Mc-3 derived from fraction Mc-1 (45 μg each), respectively, in both the panels.
