Comprehensive Evaluation of Anti-hyperglycemic Activity of Fractionated Momordica charantia Seed Extract in Alloxan-Induced Diabetic Rats
Figure 4
(a) Gel filtration chromatography of fraction Mc-3 of M. charantia: fraction Mc-3 was subjected to fractionation on a SephacrylR S-100 HR (2.5 × 80 cm) column. The figure shows a plot between fraction numbers and corresponding absorbance at 280 nm. (b) Fraction Mc-3 (15 μg, lane 1) and its fractions obtained from gel filtration chromatography were analyzed on reducing SDS-PAGE using Tris-Tricine buffer system. Lanes 2–7 represent fractions 31, 35, 39, 43, 47, and 51, respectively (indicated in panel (a)). Lane M represents migration of protein molecular weight markers.
