Comprehensive Evaluation of Anti-hyperglycemic Activity of Fractionated Momordica charantia Seed Extract in Alloxan-Induced Diabetic Rats

Table 2

Determination of the optimum dose of fraction Mc-3 for anti-hyperglycemic activity in diabetic animals.

Period (hr); of treatment	0	3	Reduction %
Normal control PBS	90±10	85±10	5.5
Diabetic control PBS	328±17.6	314±17.1	4.26
Mc-3 (5 mg/kg b.wt.)	372±74.1	287±10^∗	22.84
Mc-3 (10 mg/kg b.wt.)	301±27.5	200±18.2^∗∗∗	33.55
Mc-3 (15 mg/kg b.wt.)	541±68.5	305±43.4^∗∗∗	43.6
Mc-3 (20 mg/kg b.wt.)	474±78.9	278±42.0^∗∗∗	41.3
Mc-3 (25 mg/kg b.wt.)	368±32.1	226±25.9^∗∗∗	38.6
Insulin (10 IU/kg b.wt.)	512±39.4	288.5±33.3^∗∗∗	44

Diabetic animals were treated with different doses of fraction Mc-3. Fasting serum glucose (mg/dL) was measured before and 3 h post-administration. PBS-treated normal and diabetic animals were included as controls. Protamine zinc insulin-treated animals were included as positive controls. The data represent mean ± S.D. Each group consisted of at least 5-6 animals. ^∗𝑃<0.05; ^∗∗∗𝑃<0.001, compared with the respective group at 0 hr.